Multiple Hotspot Mutations Scanning by Single Droplet Digital PCR
نویسندگان
چکیده
منابع مشابه
Droplet Digital PCR
Introduction Genome editing tools including TALENs and the CRISPR/ Cas9 system have revolutionized our ability to edit the genome of any cell, including human induced pluripotent stem cells (iPSCs). Sequence-specific nucleases induce double-strand breaks or nicks at target sites, activating the DNA repair pathways of non-homologous end joining (NHEJ) and homology-directed repair (HDR). NHEJ pro...
متن کاملTransgene Detection by Digital Droplet PCR
Somatic gene therapy is a promising tool for the treatment of severe diseases. Because of its abuse potential for performance enhancement in sports, the World Anti-Doping Agency (WADA) included the term 'gene doping' in the official list of banned substances and methods in 2004. Several nested PCR or qPCR-based strategies have been proposed that aim at detecting long-term presence of transgene ...
متن کاملPrenatal diagnosis by droplet digital PCR.
have been tested in many lymphomas (reviewed in Blachly et al). In 2014, the US Food and Drug Administration approved a PI3K p110 d–specific inhibitor, idelalisib, for use in relapsed/refractory chronic lymphocytic leukemia and indolent lymphomas. However, this agent has failed in DLBCL phase 1 studies. Erdmann et al screened ABC and GCB cell lines with various pharmacologic agents including id...
متن کاملMultiplex Detection of Rare Mutations by Picoliter Droplet Based Digital PCR: Sensitivity and Specificity Considerations
In cancer research, the accuracy of the technology used for biomarkers detection is remarkably important. In this context, digital PCR represents a highly sensitive and reproducible method that could serve as an appropriate tool for tumor mutational status analysis. In particular, droplet-based digital PCR approaches have been developed for detection of tumor-specific mutated alleles within pla...
متن کاملQuantification of HEV RNA by Droplet Digital PCR
The sensitivity of real-time PCR for hepatitis E virus (HEV) RNA quantification differs greatly among techniques. Standardized tools that measure the real quantity of virus are needed. We assessed the performance of a reverse transcription droplet digital PCR (RT-ddPCR) assay that gives absolute quantities of HEV RNA. Analytical and clinical validation was done on HEV genotypes 1, 3 and 4, and ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2018
ISSN: 0009-9147,1530-8561
DOI: 10.1373/clinchem.2017.272518